Small RNA sequencing during salinity stress response in chickpea (small RNA-Seq)

In this study, we have identified small RNA during salinity stress response in chickpea. Small RNA library was prepared and sequencing was performed using Illumina platform. A total of 79 million reads were generated. These reads were mapped to the chickpea genome using Bowtie. Overall design: Total RNA was isolated from the control and stress samples of roots of ICCV2 and JG62 and small RNA library was prepared using TruSeq Small RNA Sample Prep Kit (Illumina Technologies) according to the manufacturer's instructions. Raw reads were obtained in FASTQ files were pre-processed to remove low-quality reads. Unique reads were mapped to the chickpea genome using Bowtie. Non-redundant set of uniquely mapped small RNA reads were retained for further processing.

本研究针对鹰嘴豆盐胁迫响应过程中的小分子RNA（small RNA）开展了鉴定工作。研究人员构建了小分子RNA文库，并采用Illumina测序平台完成测序，共产出7900万条测序读段（reads）。随后使用Bowtie工具将上述测序读段比对至鹰嘴豆参考基因组。实验设计概述：分别从ICCV2与JG62两个鹰嘴豆品种的根系对照样本及盐胁迫处理样本中提取总RNA，严格遵循制造商说明书，采用Illumina公司的TruSeq Small RNA样本制备试剂盒完成小分子RNA文库构建。以FASTQ格式存储的原始测序读段经预处理以去除低质量读段；将去重后的唯一序列读段再次通过Bowtie工具比对至鹰嘴豆参考基因组，最终保留唯一比对且非冗余的小分子RNA读段集合，用于后续分析流程。