DataSheet_3_Effective prediction of potential ferroptosis critical genes in clinical colorectal cancer.zip

BackgroundColon cancer is common worldwide, with high morbidity and poor prognosis. Ferroptosis is a novel form of cell death driven by the accumulation of iron-dependent lipid peroxides, which differs from other programmed cell death mechanisms. Programmed cell death is a cancer hallmark, and ferroptosis is known to participate in various cancers, including colon cancer. Novel ferroptosis markers and targeted colon cancer therapies are urgently needed. To this end, we performed a preliminary exploration of ferroptosis-related genes in colon cancer to enable new treatment strategies.
MethodsFerroptosis-related genes in colon cancer were obtained by data mining and screening for differentially expressed genes (DEGs) using bioinformatics analysis tools. We normalized the data across four independent datasets and a ferroptosis-specific database. Identified genes were validated by immunohistochemical analysis of pathological and healthy clinical samples.
ResultsWe identified DEGs in colon cancer that are involved in ferroptosis. Among these, five core genes were found: ELAVL1, GPX2, EPAS1, SLC7A5, and HMGB1. Bioinformatics analyses revealed that the expression of all five genes, except for EPAS1, was higher in tumor tissues than in healthy tissues.
ConclusionsThe preliminary exploration of the five core genes revealed that they are differentially expressed in colon cancer, playing an essential role in ferroptosis. This study provides a foundation for subsequent research on ferroptosis in colon cancer.

背景：结直肠癌在全球范围内高发，具有高发病率与不良预后。铁死亡（Ferroptosis）是一种由铁依赖性脂质过氧化物积累驱动的新型细胞死亡形式，与其他程序性细胞死亡机制存在显著差异。程序性细胞死亡是癌症的标志性特征之一，而铁死亡已被证实参与包括结直肠癌在内的多种癌症的发生发展。当前，临床上亟需新型铁死亡标志物与结直肠癌靶向治疗手段。为此，本研究对结直肠癌中铁死亡相关基因开展初步探索，以期为新型治疗策略的研发提供支撑。
方法：本研究借助生物信息学分析工具，通过数据挖掘与差异表达基因（differentially expressed genes, DEGs）筛选，获取结直肠癌中铁死亡相关基因；对四项独立数据集及一个铁死亡专用数据库的数据进行标准化处理；通过对病理组织与健康临床样本的免疫组织化学分析，对筛选得到的基因进行验证。
结果：本研究筛选得到了参与结直肠癌铁死亡过程的差异表达基因，其中共确定5个核心基因：ELAVL1、GPX2、EPAS1、SLC7A5及HMGB1。生物信息学分析显示，除EPAS1外，其余4个核心基因在肿瘤组织中的表达水平均高于健康组织。
结论：对上述5个核心基因的初步探索表明，它们在结直肠癌中存在差异表达，并在铁死亡过程中发挥关键作用。本研究为后续结直肠癌中铁死亡相关研究奠定了坚实基础。