Transcriptomics of BMDM treated with PTEN in the presence or absence of STAT1 inhibitors. Transcriptomics of BMDM treated with PTEN in the presence or absence of STAT1 inhibitors

PTEN binds to and regulates polarization of TAMs to enhance antitumor immunity and suppress tumor growth，We hope to investigate whether STAT1 mediates the polarization effect of PTEN on TAMs as a downstream factor Overall design: Bone-marrow (BM) cells were harvested from femurs and tibia of C57BL/6J mice. Differentiation was induced by recombinant mouse M-CSF in DMEM medium containing 1% penicillin/streptomycin and 10% FBS for 7 days to obtain the bone marrow-derived macrophages (BMDMs).To induce an M2-like phenotype, we treated BMDMs with IL-4 and IL-13 for 48 h.M2 BMDMs were treated with and without 100 ng/mL PTEN for 24 h in the presence or absence of STAT1 inhibitors (Fludarabine), followed by RNA-seq analysis.

PTEN可结合并调控肿瘤相关巨噬细胞（Tumor-associated macrophages, TAMs）的极化，以增强抗肿瘤免疫并抑制肿瘤生长。本研究拟探究STAT1作为下游因子是否介导PTEN对TAMs的极化调控作用。实验设计概述：从C57BL/6J小鼠的股骨与胫骨中收集骨髓（BM）细胞。在含有1%青霉素/链霉素与10%胎牛血清（FBS）的DMEM培养基中，使用重组小鼠巨噬细胞集落刺激因子（M-CSF）诱导分化7天，以获得骨髓来源巨噬细胞（BMDMs）。为诱导M2样表型，我们用IL-4与IL-13处理BMDMs 48小时。将M2型BMDMs在存在或不存在STAT1抑制剂氟达拉滨（Fludarabine）的条件下，用100 ng/mL PTEN处理或不处理24小时，随后进行RNA测序（RNA-seq）分析。