Spatial multi-omics reveals vulnerabilities of radial glia-like cancer-initiating cells within distinct ecosystems of pediatric and adult malignant gliomas. Spatial multi-omics reveals vulnerabilities of radial glia-like cancer-initiating cells within distinct ecosystems of pediatric and adult malignant gliomas

We collected five DMG, five GBM (including two secondary GBM), and one peritumor samples from eleven patients requiring surgical resection. To establish an atlas for spatially-resolved gene expression, we performed short-read spatial transcriptomic sequencing on the tissue sections using the 10x Visium platform. Overall design: Here, we established a spatial atlas of H3K27M-DMG and GBM through short read spatial transcriptomics to reveal the niche-specific distribution and vulnerability of stem-like cells in H3K27M-DMG and GBM

我们从11名需接受手术切除的患者体内，采集了5例弥漫中线胶质瘤（Diffuse Midline Glioma, DMG）、5例胶质母细胞瘤（Glioblastoma, GBM，含2例继发性胶质母细胞瘤）以及1例瘤旁组织样本。为构建空间解析基因表达图谱，我们使用10x Visium平台对组织切片开展短读长空间转录组测序。整体实验设计：本研究通过短读长空间转录组技术，构建了携带H3K27M突变的弥漫中线胶质瘤（H3K27M-DMG）与胶质母细胞瘤的空间图谱，旨在揭示两类肿瘤中干细胞样细胞的微环境特异性分布特征与脆弱性。