Proteomic analysis reveals changes in protein expression in oocytes at different meiosis stages

We used 4D-DIA technology to sequence the proteome of yak oocytes at four stages, germinal vesicle, germinal vesicle breakdown, meiosis I, and meiosis Ⅱ, and identified 5,851 proteins; the expression of 1,012 proteins was significantly altered in multiple stages of meiosis.我们采用4D-DIA技术对牦牛卵母细胞在四个阶段——生发泡期（germinal vesicle）、生发泡破裂期（germinal vesicle breakdown）、减数分裂I期（meiosis I）及减数分裂Ⅱ期（meiosis Ⅱ）——的蛋白质组进行测序，共鉴定出5851种蛋白质；其中1012种蛋白质的表达在减数分裂的多个阶段发生显著改变。