Genome-wide maps of nuclear lamina interactions in HeLa cells identified using MethylAdenine IDentification (MadID).. Genome-wide maps of nuclear lamina interactions in HeLa cells identified using MethylAdenine IDentification (MadID).

Mapping of lamina-associated domains using MadID experiment. A methyltransferase (M.EcoGII) targeted to the nuclear envelope is able to add methyl groups to adenine residues (m6A) on chromatin in contact to the nuclear periphery. A m6A-specific immunoprecipitation is performed on isolated genomic DNA, followed by Ilumina deep sequencing to identify contact sites.

利用MadID实验绘制核纤层结合结构域（lamina-associated domains）的图谱。靶向定位于核被膜的甲基转移酶（M.EcoGII）可在与核周区域接触的染色质的腺嘌呤残基上添加N6-甲基腺嘌呤（m6A）修饰。对分离得到的基因组DNA开展m6A特异性免疫沉淀实验，随后通过Illumina深度测序以鉴定相关接触位点。