consensus sequences drafted from microbial communities that were characterized with ONT 16S rRNA gene sequencing

Consensus sequences generated by CONCOMPRA, NGSpeciesID, and amplicon_sorter from (1) a synthetic bacterial community composed of 20 equally abundant bacteria (16S MOCK; https://www.atcc.org/products/msa-1002) or (2) the ZymoGut Microbiome Standard, a microbial community mimicking the human gut microbiome (Gut Microbiome Standard; https://files.zymoresearch.com/datasheets/ds1712_zymobiomics_gut_microbiome_standard_data_sheet.pdf). The first was sequenced twice, on both a R9 and and R10 flow cell (indicated by the folder structure). For CONCOMPRA, all the consensus sequences ('CONCOMPRA.all_consensus') are given as well as the non chimeric sequences only ('CONCOMPRA.noglobal_nolocalchim.consensus.sequences'). The full-length of the 16S rRNA gene was amplified with the primer set 27F and 1492R.
Sequencing details as well as the data processing steps are described in: Stock, Willem, et al. "Breaking free from references: a consensus-based approach for community profiling with long amplicon nanopore data." bioRxiv (2024): 2024-07. ( https://www.biorxiv.org/content/10.1101/2024.07.04.602031v1 )

由CONCOMPRA、NGSpeciesID及amplicon_sorter生成的共有序列（consensus sequences），源自两类样本：(1) 由20种丰度均等的细菌构成的合成细菌群落（16S模拟群落（16S MOCK）；https://www.atcc.org/products/msa-1002），或(2) Zymo肠道微生物组标准品（ZymoGut Microbiome Standard），一款模拟人类肠道微生物组的合成微生物群落（肠道微生物组标准品；https://files.zymoresearch.com/datasheets/ds1712_zymobiomics_gut_microbiome_standard_data_sheet.pdf）。该16S模拟群落样本曾进行两次测序，分别采用R9与R10流动池（相关信息已通过文件夹结构标注）。针对CONCOMPRA分析结果，我们同时提供了全部共有序列（'CONCOMPRA.all_consensus'）与仅含非嵌合序列的数据集（'CONCOMPRA.noglobal_nolocalchim.consensus.sequences'）。16S核糖体RNA（16S rRNA）基因的全长序列通过引物对27F与1492R完成扩增。
测序细节与数据处理步骤详见文献：Stock, Willem 等. “脱离参考序列：基于共有序列的长扩增子纳米孔测序群落分析方法”. bioRxiv (2024): 2024-07. (https://www.biorxiv.org/content/10.1101/2024.07.04.602031v1)