RNA-seq of postnatal day 0 (P0) wild-type and Satb2-/- cortices

The goal of the study was to compare gene expression of P0 wild-type and P0 Satb2-/- cortices. Total RNAs were isolated from P0 cortices dissected from wild-type and Satb2-/- mice (n=3 for each genotype), following Qiagen RNAeasy kit instruction.Sequence libraries were made following Illumina RNA TruSeq library preparation guide.The libraries were pair-end sequenced (50nt per end). Differentially expressed genes were identified by DESEQ. Total RNAs were isolated from P0 cortices (3 control and 3 mutants), and sequenced on Illumina Genome Analyzer本研究旨在比较P0龄野生型（wild-type）与P0龄Satb2-/-小鼠大脑皮层的基因表达水平。严格遵循Qiagen RNAeasy试剂盒说明书的操作流程，从野生型及Satb2-/-小鼠（每种基因型样本数n=3）解剖分离的P0龄大脑皮层中提取总RNA。参照Illumina RNA TruSeq文库制备指南构建测序文库，随后对文库进行双端测序，每端读长为50nt。通过DESEQ软件鉴定差异表达基因。此外，从P0龄大脑皮层（3例对照样本、3例突变样本）中提取总RNA，并在Illumina Genome Analyzer上完成测序。