Yeast gene deletions that lead to the formation of supersized LDs (SLDs).

Cells were grown in synthetic complete (SC) media or rich YPD media to stationary phase, stained with Nile red, and observed under a fluorescence microscope for LDs. Experiments were done in triplicates and ∼200 cells were counted for all strains each time. The percentages of cells that displayed SLDs were represented as mean±SD. SC has 11 µM inositol because it contains yeast nitrogen base.

细胞于合成完全（SC）培养基或富营养YPD培养基中培养至稳定期，经尼罗红（Nile red）染色后，通过荧光显微镜观察脂滴（LDs）。实验设置三次重复，每次实验均对所有供试菌株计数约200个细胞。呈现应激脂滴（SLDs）的细胞占比以平均值±标准差（mean±SD）表示。合成完全（SC）培养基的肌醇浓度为11 μM，因其配方中含有酵母氮源基础培养基（yeast nitrogen base）。