The Effect of Feed Enriched with DHA and EPA Using Fish Oil on Fatty Acid Content and Growth of Asian Redtail Catfish <i>Hemibagrus nemurus</i> for Food Supply

This study aims to determine the effect of enriching feed with EPA (eicosapentaenoic acid) + DHA (docosahexaenoic acid) using fish oil on fatty acids content, whole-body carcass, growth, and feed efficiency in Asian redtail catfish Hemibagrus nemurus. The test samples comprised 180 juveniles weighing 35.29 ±0.75 g, which were divided into 4 groups. In addition, the samples were raised in 12 circular tarpaulin ponds, each containing 200 liters of water and 15 fish. The experiment consisted of 3 replicates, and feed was enriched with varying levels of EPA+DHA, namely 0 mg/kg (A1), 6,300 mg/kg (A2), 9,180 mg/kg (A3), and 12,600 mg/kg (A4). Fish were fed a daily portion equivalent to 5% of body weight, followed by analyses. The results showed that the enrichment of feed with varying levels of EPA+DHA using fish oil significantly influenced fatty acid compositions and whole-body carcass, as well as growth and feed efficiency (p&lt;0.05). The total n-3 content of feed increased from 5.55% to 8.46%, while the whole-body carcass experienced an increment from 2.66% to 3.45%. In addition, the total n-6 content decreased from 20.38% to 18.95%, with whole-body carcass experiencing a decrease from 15.53% to 10.56%. The results also showed that the DHA levels increased from 1.19% to 1.67% of the total fatty acids. In A4, the C20:5 n -3 (EPA) levels increased from 0.62 to 0.90%, while the C22:6 n-3 (DHA) levels increased from 1.19 to 1.93%. The final body weight and feed conversion of fish increased from 82.50 g to 104.50 g and 80.04% to 94.36%, respectively, while the lipid retention decreased from 152.38% to 133.51%. Based on these results, enrichment with 12,600 mg EPA+DHA using fish oil had proven to be beneficial in improving growth, fatty acids in carcass, and feed efficiency of Hemibagrus nemurus.本研究旨在探究以鱼油富集二十碳五烯酸（EPA, eicosapentaenoic acid）+二十二碳六烯酸（DHA, docosahexaenoic acid）的饲料，对亚洲红尾鲶（Hemibagrus nemurus）的脂肪酸组成、全鱼体成分、生长性能及饲料利用效率的影响。试验样本为180尾初始体重35.29±0.75 g的幼鱼，随机分为4组。实验设置12个圆形篷布养殖池，每池水体体积200 L，放养15尾幼鱼，设3个生物学重复。各组饲料以鱼油添加不同梯度的EPA+DHA，分别为0 mg/kg（A1组）、6300 mg/kg（A2组）、9180 mg/kg（A3组）及12600 mg/kg（A4组）。每日投喂量为鱼体体重的5%，养殖周期结束后开展各项指标检测。结果显示，不同梯度鱼油源EPA+DHA富集饲料对亚洲红尾鲶的脂肪酸组成、全鱼体成分、生长性能及饲料利用效率均具有显著影响（p<0.05）。饲料中总n-3多不饱和脂肪酸含量从5.55%提升至8.46%，全鱼体脂含量从2.66%升高至3.45%；同时饲料总n-6多不饱和脂肪酸含量从20.38%降至18.95%，全鱼体脂n-6含量从15.53%下降至10.56%。此外，饲料总脂肪酸中DHA占比从1.19%提升至1.67%；在A4组中，C20:5 n-3（EPA）占比从0.62%升至0.90%，C22:6 n-3（DHA）占比从1.19%升至1.93%。试验鱼终末体重从82.50 g提升至104.50 g，饲料转化率从80.04%升高至94.36%，而脂肪保留率从152.38%降至133.51%。基于上述结果，以12600 mg/kg剂量的鱼油源EPA+DHA富集饲料，可有效改善亚洲红尾鲶的生长性能、体脂肪酸组成及饲料利用效率。