Flye assemblies (long-read only assemblies)
收藏DataCite Commons2025-05-26 更新2024-07-28 收录
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https://figshare.com/articles/dataset/Flye_assemblies_long-read_only_assemblies_/14706015
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Flye (version 2.8.3-b1695) [31] was run with all the passed ONT reads (phred score > 7) available per barcode (--nano-raw), specifying the option to recover plasmids (--plasmids), indicating an expected genome size of 5 Mbp (--genome-size 5m) and considering 3 polishing rounds (--iterations 3). <br>ONT reads were demultiplexed and basecalled using Guppy (version 4.2.3). <br>
Flye(版本2.8.3-b1695)[31] 针对每个条形码对应的所有Phred质量值大于7的合格ONT(Oxford Nanopore Technologies,牛津纳米孔测序)读段,采用--nano-raw参数运行,开启恢复质粒的配置选项(--plasmids),设置预期基因组大小为5 Mbp(--genome-size 5m),并配置3轮抛光迭代(--iterations 3)。
ONT读段已通过Guppy(版本4.2.3)完成分样与碱基识别。
提供机构:
figshare
创建时间:
2021-05-31



