Transcriptome analysis of endometriotic stromal cells treated with hydrogen peroxide

In ovarian endometrioma, much iron, which is derived from blood in cyst, deposit in stroma. This iron generates oxidative stress by Fenton reaction, which is supposed to affect endometriotic stromal cells. We used gene expression microarrays to find influence of oxidative stress on endometriotic stromal cells. Gene expression microarrays revealed no statistically differences caused by oxidative stress. 9 samples, which were 3 immortalized eutopic endometrial cells from patients without endometriosis, 3 immoralized eutopic endometrial cells from patients with ovarian endometrioma and 3 immortalized endometriotic stromal cells from patients with ovarian endometrioma, were treated with 1uM hydrogen peroxide for 60 hours and 9 controls untreated with hydrogen peroxide were prepared. A total of 18 microarrays were used for 9 samples treated with hydrogen peroxide and 9 controls.

在卵巢子宫内膜异位囊肿（ovarian endometrioma）病灶内，源自囊腔积血的大量铁元素会沉积于间质组织中。该铁元素可通过芬顿反应（Fenton reaction）诱发氧化应激，被认为会对异位间质细胞产生影响。本研究借助基因表达微阵列（gene expression microarrays）技术，探究氧化应激对异位间质细胞的影响。基因表达微阵列分析结果显示，氧化应激未引发具有统计学意义的基因表达差异。本次实验共设置9份处理样本与9份未施加过氧化氢（hydrogen peroxide）的对照样本：处理样本包含3株来自无子宫内膜异位症患者的永生化（immortalized）在位子宫内膜细胞（eutopic endometrial cells）、3株来自卵巢子宫内膜异位囊肿患者的永生化在位子宫内膜细胞，以及3株来自同一类患者的永生化异位间质细胞（endometriotic stromal cells），所有处理样本均以1μM浓度的过氧化氢处理60小时。共计使用18张基因表达微阵列，分别对应9份过氧化氢处理样本与9份对照样本。