RNA-sequencing Analysis (RNA-seq)，Genome-wide Maps of Chromatin State (ChIP-seq) and Assay for Transposase Accessible Chromatin with High-throughput Sequencing (ATAC-seq) in BMDMs or Raw 264.7 cell lines.

For RNA-seq,mRNA profiles of Bone marrow derived macrophages(BMDMs) of wild-type(WT) and Zmynd8 knockout(Zmynd8 cKO),p65 KO + p65,p65 KO + K122R in Raw264.7 cells,Zmynd8 KO + Zmynd8,Zmynd8 KO + D/E-A in Raw264.7 cells were generated from GUANGZHOU RIBOBIO,using Illumina Hiseq.The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: Burrows–Wheeler Aligner (BWA) followed by ANOVA (ANOVA) and TopHat followed by Cufflinks. qRT–PCR validation was performed using TaqMan and SYBR Green assays.For ChIP-seq,BMDMs or Raw264.7 cells (about 1x107 cells) were fixed with 1% methanol-free formaldehyde at room temperature for 10 min, followed by quenching with 125mM glycine,and chromatin immunoprecipitated DNA was send to Novogene,using Nova-PE150.The FASTQ data were mapped to the mouse genome (mm10) using Bowtie, and significant enrichments were identified by MACS2.0 using Broad Peak mode with p-value ≤1*10 -5, FDR ≤ 0.01 as a cutoff to call peaks from the aligned results.For ATAC-seq,BMDMs were send to Shanghai Da Che Biotechnology Company,and the FASTQ data were mapped to the mouse genome (mm10) using Bowtie, and significant enrichments were identified by MACS2.0 using Broad Peak mode with p-value ≤1*10 -5, FDR ≤ 0.01 as a cutoff to call peaks from the aligned results. mRNA profiles of Bone marrow derived macrophages(BMDMs) of wild-type(WT) and Zmynd8 knockout(Zmynd8 cKO),p65 KO + p65,p65 KO + K122R in Raw264.7 cells,Zmynd8 KO +Zmynd8,Zmynd8 KO + D/E-A in Raw264.7 cells .For ChIP-seq,examination of ZMYND8 ,LSD1,H3K4me1 binding peaks in BMDMs,and ZMYND8 binding peaks in p65 KO,p65 KO + p65,p65 KO +K122R Raw264.7 cells ,Zmynd8 KO,Zmynd8 KO + Zmynd8,Zmynd8 KO + D/E-A Raw264.7 cells.

本数据集涵盖三类组学测序数据，具体如下：
1. RNA测序（RNA-seq）：包含野生型（WT）和Zmynd8敲除（Zmynd8 knockout, Zmynd8 cKO）的骨髓源性巨噬细胞（Bone marrow derived macrophages, BMDMs），以及Raw264.7细胞中p65敲除（p65 KO）+ p65、p65敲除 + K122R突变、Zmynd8敲除 + Zmynd8、Zmynd8敲除 + D/E-A组的mRNA表达谱。该测序由广州RIBOBIO（GUANGZHOU RIBOBIO）依托Illumina Hiseq平台完成。经质量过滤的序列读数在转录本异构体水平采用两种方案分析：一是Burrows–Wheeler Aligner（BWA）结合方差分析（ANOVA），二是TopHat结合Cufflinks。后续通过TaqMan和SYBR Green分析法完成qRT-PCR验证。
2. 染色质免疫沉淀测序（ChIP-seq）：
- 样本处理与测序：将BMDMs或Raw264.7细胞（约1×10⁷个）用1%无甲醇甲醛室温固定10分钟，以125mM甘氨酸终止固定反应；染色质免疫沉淀获取的DNA送至诺禾致源（Novogene），采用Nova-PE150进行测序。
- 数据分析：FASTQ数据通过Bowtie比对至小鼠基因组mm10版本，使用MACS2.0以Broad Peak模式、p值≤1×10⁻⁵且错误发现率（FDR）≤0.01为阈值，从比对结果中调用显著富集峰。
- 检测靶点：涵盖BMDMs中ZMYND8、LSD1、H3K4me1的结合峰检测，以及p65敲除、p65敲除 + p65、p65敲除 + K122R的Raw264.7细胞，Zmynd8敲除、Zmynd8敲除 + Zmynd8、Zmynd8敲除 + D/E-A的Raw264.7细胞中ZMYND8的结合峰检测。
3. 转座酶可及性测序（ATAC-seq）：BMDMs样本送至上海达驰生物技术有限公司，其FASTQ数据通过Bowtie比对至小鼠基因组mm10版本，使用MACS2.0以Broad Peak模式、p值≤1×10⁻⁵且FDR≤0.01为阈值，从比对结果中调用显著富集峰。