Chlorella a genus of microalgae has gained attention as a biofertilizer due to its beneficial effects on Plant Growth Promoting Rhizobacteria. This study evaluates the biostimulant effect of Chlorella liquid biofertilizer on Coriandrum sativum by analyzing microbial diversity in treated soil. Genomic DNA from soil samples is analyzed through 16S rRNA sequencing to assess microbial composition. The analysis reveals Acinetobacter rhizosphaerae and Pseudomonas fragi as dominant species with Acinetobacter rhizosphaerae comprising 25 percent of the total abundance and promoting plant growth. Other species like Brevundimonas vesicularis and Lysobacter brunescens are also identified highlighting the biofertilizers impact on soil microbial diversity.

Chlorella a genus of microalgae has gained attention as a potential biofertilizer due to its unique characteristics and beneficial effects on Plant Growth Promoting Rizobacteria. The study aims to evaluate the biostimulant effect of the Chlorella liquid biofertilizer on Coriandrum sativum assessing its ability to enhance plant growth productivity and overall health. This study focuses on the isolation and identification of Chlorella spp. and the subsequent production of a liquid biofertilizer. The objective is to investigate the biostimulant effect of Chlorella liquid biofertilizer on Coriandrum sativum plants. To assess the impact of the Chlorella liquid biofertilizer genomic DNA is extracted from the soil samples of the coriander crop treated with the biofertilizer. Library preparation is conducted for the biostimulant Coriandrum sativum crop soil and 16S rRNA gene sequencing data analysis is performed. This analysis includes quality control of the raw sequencing data Operational Taxonomic Unit classification and alpha diversity analysis to explore the microbial community composition and diversity in the soil. By examining the alpha diversity of the soil microbial community this research aims to uncover any potential correlations between the application of Chlorella liquid biofertilizer and changes in the microbial diversity and composition within the soil. The findings from this study will provide valuable insights into the microbial analysis of the soil samples revealed the presence of various species with Acinetobacter Rhizosphaerae and Pseudomonas Fragi being the most dominant. Acinetobacter Rhizosphaerae accounted for approximately 25 percentage of the total abundance followed closely by Pseudomonas Fragi at 20 percentage. Other species such as Brevundimonas Vesicularis Hydrogenophaga Palleronii Lysobacter Brunescens Peredibacter Starri Phaselicystis Flava Pseudoxanthomonas Mexicana Terribacillus Halophilus Woodsholea Maritima and an unidentified species were also present representing 10 percentage of the total abundance. The results reveals the presence of Acinetobacter Rhizosphaerae promotes growth of plant.

小球藻（Chlorella）作为一类微藻（microalgae），凭借其独特的生物学特性以及对植物促生根际细菌（Plant Growth Promoting Rhizobacteria）的有益作用，作为潜在生物肥料（biofertilizer）受到广泛关注。本研究旨在评估小球藻液体生物肥料对芫荽（Coriandrum sativum）的生物刺激素效应，探究其促进植物生长、提升产量与整体健康水平的能力。本研究聚焦于小球藻属（Chlorella spp.）菌株的分离与鉴定，以及后续液体生物肥料的制备，核心目标为探究小球藻液体生物肥料对芫荽植株的生物刺激素效应。为评估该小球藻液体生物肥料的施用效果，研究人员从施加了该生物肥料的芫荽作物土壤样本中提取基因组DNA（genomic DNA），随后完成该生物刺激素处理组芫荽作物土壤的文库构建（library preparation），并开展16S rRNA基因测序数据分析。该分析流程涵盖原始测序数据质控、操作分类单元（Operational Taxonomic Unit，OTU）分类以及α多样性分析，以解析土壤微生物群落的组成结构与多样性特征。本研究通过分析土壤微生物群落的α多样性，旨在揭示小球藻液体生物肥料的施用与土壤微生物多样性及群落组成变化之间的潜在关联。本研究的土壤样本微生物分析结果显示，样本中存在多种微生物类群，其中不动杆菌（Acinetobacter）、根球孢菌（Rhizosphaerae）与脆弱假单胞菌（Pseudomonas Fragi）为优势类群。不动杆菌（Acinetobacter）约占总丰度的25%，脆弱假单胞菌（Pseudomonas Fragi）紧随其后，占比约20%。其余类群包括泡囊短波单胞菌（Brevundimonas Vesicularis）、帕拉氢噬胞菌（Hydrogenophaga Palleronii）、棕色溶杆菌（Lysobacter Brunescens）、斯特里噬纤维菌（Peredibacter Starri）、黄色类囊泡菌（Phaselicystis Flava）、墨西哥假黄色单胞菌（Pseudoxanthomonas Mexicana）、嗜盐地芽孢杆菌（Terribacillus Halophilus）、海滨沃氏杆菌（Woodsholea Maritima）以及1株未鉴定微生物类群，合计约占总丰度的10%。本研究结果表明，不动杆菌（Acinetobacter）的存在可有效促进植物生长。