Supplemental Material for Zhao et al., 2020

Supporting information was consist of Supplementary Figures and Supplementary Tables, available at figshare. Figure S1 describes RNA-seq analysis of ZS9 and yvl. Figure S2 shows polymorphic molecular markers. Figure S3, S4 and S5 illustrate sequence alignment of three genes in which SNP variation occurred. Figure S6 and S7 illustrate amplification and digestion by Bln I of ORF10. Figure S8 illustrates the domain of CHLH protein in multiple species. Figure S9 shows FPKM value of BnaA03.CHLH in ZS11 tissues. Figure S10 illustrates the expression of chlorophyll synthesis genes. Table S1 lists the information of 629 rapeseed accessions. Table S2 describes all the primer sequence in this study. Table S3 describes the comparison of agronomic traits between ZS9 and yvl mutant. Table S4 describes chlorophyll contents of younger and older leaves between ZS9 and yvl. Table S5 shows the statistics of whole genome re-sequencing in BSA-seq study. Table S6 and S7 respectively list the functional annotation of candidate genes and genes transcribed by PEP and NEP.

本研究的辅助信息包含补充图与补充表，可于figshare平台获取。补充图S1展示了ZS9与yvl的RNA测序（RNA-seq）分析结果；补充图S2呈现了多态性分子标记；补充图S3、S4及S5展示了发生单核苷酸多态性（SNP）变异的三个基因的序列比对结果；补充图S6与S7展示了ORF10的扩增产物经Bln I酶切的实验结果；补充图S8呈现了多个物种中CHLH蛋白的结构域信息；补充图S9展示了ZS11各组织中BnaA03.CHLH的每百万片段每千碱基序列读数（FPKM）值；补充图S10展示了叶绿素合成相关基因的表达模式。补充表S1列出了629份油菜种质资源的基本信息；补充表S2收录了本研究中使用的全部引物序列；补充表S3对比了ZS9与yvl突变体的农艺性状差异；补充表S4对比了ZS9与yvl突变体幼叶及老叶的叶绿素含量；补充表S5展示了群体分离分析测序（BSA-seq）研究中的全基因组重测序统计数据；补充表S6与S7分别列出了候选基因的功能注释信息，以及由质体编码RNA聚合酶（PEP）和核编码RNA聚合酶（NEP）转录的基因列表。