Expression data from human healthy and lupus EPCs/CACs, and healthy CD133+ bone marrow EPCs

Systemic lupus erythematosus (SLE) is characterized by increased vascular risk due to premature atherosclerosis independent of traditional risk factors. We previously proposed that interferon-α plays a crucial role in premature vascular damage in SLE. IFN-α alters the balance between endothelial cell apoptosis and vascular repair mediated by endothelial progenitor cells (EPCs) and myeloid circulating angiogenic cells (CACs). Here we demonstrate that IFN-α promotes an antiangiogenic signature in SLE and control EPCs/CACs, characterized by transcriptional repression of IL-1α and β, IL-1 receptor 1 and vascular endothelial growth factor A (VEGF-A) and upregulation of IL-1 receptor antagonist (IL-1RN) and the decoy receptor IL1-R2. IL-1β promotes significant improvement in the functional capacity of lupus EPCs/CACs, therefore abrogating the deleterious effects of IFN-α. We used microarrays to analyze the effect of IFNα on peripheral blood EPCs/CACs and on bone marrow EPCs exposed to proangiogenic stimulation. This SuperSeries is composed of the SubSeries listed below. Overall design: Human healthy and lupus EPCs and CACs from PBMCs, and healthy EPCs from bone marrow, were isolated and cultured under proangiogenic stimulation; after IFN-α incubation or not, RNA was extracted and processed for hybridization on Affymetrix microarrays.

系统性红斑狼疮（Systemic lupus erythematosus, SLE）以独立于传统危险因素的早发性动脉粥样硬化所致血管风险升高为特征。我们此前提出，α干扰素（interferon-α, IFN-α）在系统性红斑狼疮的早发性血管损伤中发挥关键作用。α干扰素可改变内皮细胞凋亡与内皮祖细胞（endothelial progenitor cells, EPCs）、髓系循环血管生成细胞（myeloid circulating angiogenic cells, CACs）介导的血管修复之间的平衡。本研究证实，α干扰素可在系统性红斑狼疮中促成抗血管生成特征，并调控内皮祖细胞/循环血管生成细胞，具体表现为对白细胞介素1α、白细胞介素1β、白细胞介素1受体1及血管内皮生长因子A（vascular endothelial growth factor A, VEGF-A）的转录抑制，以及对白细胞介素1受体拮抗剂（IL-1 receptor antagonist, IL-1RN）与诱饵受体IL1-R2的上调表达。白细胞介素1β可显著改善狼疮患者内皮祖细胞/循环血管生成细胞的功能活性，从而抵消α干扰素的有害作用。我们利用微阵列技术分析了α干扰素对外周血内皮祖细胞/循环血管生成细胞，以及经促血管生成刺激的骨髓内皮祖细胞的影响。本超级数据集由以下所列的子数据集构成。实验设计概述：分离并培养来自外周血单个核细胞（peripheral blood mononuclear cells, PBMCs）的健康人及狼疮患者内皮祖细胞、循环血管生成细胞，以及骨髓来源的健康人内皮祖细胞，所有样本均在促血管生成刺激条件下培养；经α干扰素孵育或不予孵育后，提取RNA并在Affymetrix微阵列上完成杂交检测。