Analysis of lncrna expression profile in LPS activated THP1 cells

In order to further explore the expression of lncrna in asthma, we used lipopolysaccharide (LPS) to activate human monocyte macrophage THP-1, and then used deep sequencing method to detect the expression of lncrna in activated and inactive THP1 cells. The results showed that there were different expression of lncrna in LPS activated and inactive THP1 cells Control and LPS treated THP-1 cells for 48h were collected in Trizol plus reagent. The sample total RNA and lncRNA sequencing were performed by Boao Biotech in Guangzhou, China

为进一步探索长链非编码RNA（lncRNA）在哮喘中的表达，我们采用脂多糖（lipopolysaccharide, LPS）激活人单核巨噬细胞THP-1，随后通过深度测序技术检测激活与未激活状态下THP-1细胞中lncRNA的表达水平。研究结果显示，经LPS激活与未激活的THP-1细胞中lncRNA的表达存在差异。将培养48小时的对照组与LPS处理组THP-1细胞收集至Trizol Plus试剂中。本次实验的样本总RNA提取及lncRNA测序工作由中国广州的博奥生物科技（Boao Biotech）完成。