Single-cell transcriptome dynamics in stabilized pituitary gonadotrope cells [bulk_pituitary]

Studying dynamic transcripts in single cells (SC) requires large numbers of timed samples. We report an easy to use protocol to stabilize RNA in intact SCs without perturbing transcriptional patterns, and demonstrate its applicability for SC transcriptome assays with cells and tissue. We identify a gene-specific hierarchical pattern of all-or-none transcript induction elicited by different concentrations of pulsatile hormone stimuli in pituitary gonadotropes. Overall design: Bulk RNA-seq was performed on two primary mouse pituitary cell samples, one stored in RNA-Best, the other in RNAlater (reference sample).针对单细胞（single cell, SC）动态转录本的研究，需要获取大量时序样本。本研究报道了一种操作简便的实验方案，可在不干扰转录模式的前提下，稳定完整单细胞内的RNA，并验证了该方案可应用于细胞及组织样本的单细胞转录组检测。本研究还鉴定出，在垂体促性腺激素细胞中，不同浓度脉冲式激素刺激可诱导转录本呈现全或无的表达模式，且该模式具有基因特异性的层级特征。实验设计总览：本研究对两份原代小鼠垂体细胞样本进行了批量RNA测序（Bulk RNA-seq）：其中一份样本采用RNA-Best保存，另一份以RNAlater保存作为对照样本。