OMAP-5 Organ Mapping Antibody Panel (OMAP) for Multiplexed Antibody-Based Imaging of Human Liver with SIMS, v1.2

OMAP-5 was designed for Secondary Ionization Mass Spectrometry (SIMS) imaging of fresh frozen human liver samples<br>(https://doi.org/10.1101/2022.09.26.508878). The panel contains 20 antibodies and a cationic nucleic acid intercalator<br>that contains natural-abundance iridium. An antibody directed against Na/K ATPase was used for cell segmentation and<br>image alignment. This OMAP provides spatial context for the anatomical structures, metabolic zonation, and cell types<br>present in the [ASCT+B liver table v1.0](https://doi.org/10.48539/HBM476.BQCC.574). This OMAP does not yet contain<br>antibodies for NK cells, dendritic cells, portal fibroblasts, MAIT cells, gamma delta T cells, and hepatic oval cells.<br>Antigens were detected using lanthanide tagged primary antibodies where the metal tags were detected by specific mass<br>channels using C60 SIMS. Details on the antibody staining method can be found here<br>(dx.doi.org/10.17504/protocols.io.b5qmq5u6). Image registration and cell segmentation were performed using Deepcell<br>(Keren et al.) Cell segmentation was performed using nuclear and membrane marker channels. Integrated protein<br>expression was used to determine thresholds for cellular classification. An antibody directed against EGFR was<br>originally included in OMAP-5 v1.0 and v1.1, but was removed due to inconsistent labeling.

OMAP-5 是为新鲜冷冻人肝脏样本的二次离子质谱（Secondary Ionization Mass Spectrometry, SIMS）成像实验设计的数据集（https://doi.org/10.1101/2022.09.26.508878）。
该抗体组合包含20种抗体与一种搭载天然丰度铱的阳离子核酸嵌入剂。
针对钠钾ATP酶（Na/K ATPase）的抗体被用于细胞分割与图像配准。
该OMAP可为[ASCT+B肝脏表格v1.0]（https://doi.org/10.48539/HBM476.BQCC.574）中收录的解剖结构、代谢分区及细胞类型提供空间分布信息。
当前OMAP-5尚未包含针对自然杀伤（NK）细胞、树突状细胞、门脉成纤维细胞、黏膜相关恒定T（MAIT）细胞、γδ T细胞以及肝卵圆细胞的抗体。
实验采用镧系元素标记的一抗检测抗原，通过C60 SIMS的专属质量通道识别金属标签。
抗体染色方法的详细信息可参见（dx.doi.org/10.17504/protocols.io.b5qmq5u6）。
图像配准与细胞分割均依托Deepcell（Keren等人）完成，细胞分割基于细胞核与细胞膜标志物通道实现。
通过整合蛋白表达量确定细胞分类的阈值。
最初的OMAP-5 v1.0与v1.1版本中包含了针对表皮生长因子受体（EGFR）的抗体，但因标记结果不一致已被移除。