Differentiation-dependent changes in the epigenomic landscape of human myeloid leukemia cells implicate signaling pathways and highlight chromosomal amplifications. Homo sapiens

To assess how DNA modification patterns are influenced by myelopoesis, we have mapped regions enriched for 5mC or 5hmC throughout the genome during promyelocyte to granulocyte differentiation in the HL-60 cell model. Overall design: MeDIP-seq and hMeDIP-seq were performed on undifferentiated and 1.5% DMSO differentiated HL-60 cells.

为评估髓系造血过程如何影响DNA修饰模式，我们在HL-60细胞模型中，对早幼粒细胞向粒细胞分化的全基因组范围内富集5-甲基胞嘧啶（5mC）或5-羟甲基胞嘧啶（5hmC）的区域进行了定位分析。实验设计：我们对未分化以及经1.5%二甲基亚砜（DMSO）诱导分化的HL-60细胞，分别开展了甲基化DNA免疫沉淀测序（MeDIP-seq）与羟甲基化DNA免疫沉淀测序（hMeDIP-seq）。