VMP1 restricts NLRP3 inflammasome activation through its modulation of SERCA activity and autophagy

To investigate the effect of VMP1 deletion on gene expression in either untreated or LPS and ATP treated human differentiated THP-1 cells. CRISPR-Cas9 technology was used to generate stable knockout cell lines. We then performed an RNA-Seq experiment to assess gene expression changes between untreated and treated VMP1 KO or control macrophages. Overall design: Comparative gene expression profiling analysis of RNA-seq data for THP-1 cells and its KO derivatives (VMP1 KO). Three replicates were included for each condition: untreated differentiated control THP-1s, LPS and ATP treated differentiated control THP-1s, untreated differentiated VMP1 KO THP-1s, and LPS and ATP treated differentiated VMP1 KO THP-1s.

本研究旨在探究VMP1敲除（VMP1 knockout, KO）对未处理或经脂多糖（LPS）与三磷酸腺苷（ATP）处理的人源分化THP-1细胞基因表达的影响。本研究采用CRISPR-Cas9技术构建稳定敲除细胞系，随后开展RNA测序（RNA-Seq）实验，以评估未处理与经处理的VMP1敲除巨噬细胞与对照巨噬细胞间的基因表达变化。整体实验设计：针对THP-1细胞及其VMP1敲除衍生细胞系的RNA测序数据进行比较基因表达谱分析。每种实验条件设置三个重复，具体包括：未处理的分化型对照THP-1细胞、经LPS与ATP处理的分化型对照THP-1细胞、未处理的分化型VMP1敲除THP-1细胞，以及经LPS与ATP处理的分化型VMP1敲除THP-1细胞。