Gene expression profiles of acetaminophen, isoniazid, and paraquat treated C57BL/6 mice. Mus musculus

Toxicogenomics (tgx) is used as a tool to identify mechanisms and markers of necrosis in C57BL/6 mice treated by oral gavage using acetaminophen (APAP), isoniazid (INZ), and paraquat (PQ). Critical doses for tgx analysis were derived from a 25 day dose range finding study. For tgx analysis, livers of mice were collected 1 and 2 days after a single compound dose of 168.75, 225, and 300 mg/kg bw for APAP; 12.5, 25, and 50 mg/kg bw for PQ; and 22, 44, and 88 mg/kg bw for INZ. All samples were diluted in water. Overall design: For the toxicogenomics study, samples were as followed: five mice per low, medium, and high dose group at 1 day, and five mice in the high dose group at 2 days. One set of vehicle controls (n=5) per day was used. The total number of samples was 70.

毒理基因组学（Toxicogenomics，tgx）作为研究工具，用于鉴定经灌胃给予对乙酰氨基酚（acetaminophen，APAP）、异烟肼（isoniazid，INZ）及百草枯（paraquat，PQ）的C57BL/6小鼠的坏死相关机制与标志物。用于毒理基因组学分析的关键剂量源自一项为期25天的剂量范围探索实验。在毒理基因组学分析中，针对对乙酰氨基酚设置168.75、225、300 mg/kg体重的单次给药剂量，百草枯设置12.5、25、50 mg/kg体重的单次给药剂量，异烟肼设置22、44、88 mg/kg体重的单次给药剂量，分别于给药后1天和2天采集小鼠肝脏样本，所有样本均经水溶液稀释。本毒理基因组学研究的样本分组如下：给药后1天时，低、中、高剂量组各取5只小鼠；给药后2天时，仅高剂量组取5只小鼠；每日设置1组溶媒对照组（n=5）。本研究总样本量为70。