Array CGH analysis of human colorectal tumors. Homo sapiens

The concept of the CpG island methylator phenotype (CIMP) in colorectal cancers (CRCs) is widely accepted, though the timing of its occurrence and its interaction with other genetic defects early during carcinogenesis remains largely unknown. Our aim was to uncover the molecular evolution of CIMP CRCs through integrative analysis of endoscopic, histological and molecular signatures in precancerous and malignant colorectal lesions. Overall design: A total of 84 endoscopically obtained human colorectal tumor was analyzed using Agilent CGH microarray. Copy number aberration was compared with clinicopathological features and DNA methylation status.

结直肠癌（colorectal cancers, CRCs）中CpG岛甲基化表型（CpG island methylator phenotype, CIMP）的概念已获广泛认可，但其发生时序以及癌变早期与其他遗传缺陷的相互作用仍未完全阐明。本研究旨在通过对癌前结直肠病变与恶性结直肠病变的内镜、组织学及分子特征开展整合分析，揭示CIMP阳性结直肠癌的分子演化规律。实验整体设计：本研究共纳入84例经内镜获取的人类结直肠肿瘤样本，采用安捷伦比较基因组杂交微阵列（Agilent CGH microarray）开展检测；将拷贝数变异与临床病理特征及DNA甲基化状态进行对比关联分析。