RNA sequencing Facilitates Quantitative Analysis of ANGPTL8 on Lipid metabolism in liver of chow mice

We report the use of transcriptome sequencing in the intervention of ANGPTL8 in the regulation of triglyceride level. We constructed the adenovirus-mediated overexpression and knockdown of ANGPTL8 in wild-type mice and obtained the differential genes of ANGPTL8 regulating serum triglyceride level by transcriptomic sequencing. We identified 234 differentially expressed ANGPTL8 response genes, 113 genes are up-regulated in ANGPTL8 overexpression,and down-regulated in ANGPTL8 knock-down. 121 genes are up-regulated in ANGPTL8 down-knock, and down-regulated in ANGPTL8 overexpression. This study provides a framework for the network regulation mechanism of ANGPTL8 controlling triglyceride level. Overall design: Exploring the Signal Network of ANGPTL8 Regulating Lipid Metabolism Through RNA sequencing

本研究报道了转录组测序在血管生成素样蛋白8（ANGPTL8）调控甘油三酯水平干预实验中的应用。本研究在野生型小鼠中构建了腺病毒介导的ANGPTL8过表达与敲低模型，并通过转录组测序获取了ANGPTL8调控血清甘油三酯水平的差异表达基因。本研究共鉴定出234个ANGPTL8应答差异表达基因：其中113个基因在ANGPTL8过表达组中上调，在ANGPTL8敲低组中下调；另有121个基因在ANGPTL8敲低组中上调，在ANGPTL8过表达组中下调。本研究为ANGPTL8调控甘油三酯水平的网络调控机制提供了研究框架。整体实验设计：通过RNA测序探究ANGPTL8调控脂质代谢的信号网络。