Next Generation Sequencing Facilitates Quantitative Analysis of Dura macrophage Transcriptomes post adoptive T cell reconstitution of RAG-1 deficient mice I

The goal of this study was to compare transcriptional changes evoked by T cells cross talk with meningeal macrophages. Furthermore, the role of specific T cell subsets or selected cytokine deficiency were used to evaluate their role in regulating such transcriptional changes Dural macrophage mRNA profiles post T cell reconstitution of RAG-1 deficient mice were generated by deep sequencing, in replicate, using Illumina HiSeq

本研究旨在比较T细胞与脑膜巨噬细胞（meningeal macrophages）之间的细胞串扰所诱导的转录变化。此外，本研究通过特定T细胞亚群或选择性细胞因子缺陷模型，评估其在调控上述转录变化中的作用。本研究采用Illumina HiSeq平台，对RAG-1缺陷小鼠经T细胞重建后的硬脑膜巨噬细胞（dural macrophage）mRNA表达谱进行了带有生物学重复的深度测序。