Transcriptomic profiling of circadian genes in WT mouse tissues (mRNA-Seq)

A goal of a collaboration between the CHDI Foundation and Prof. Jenny Morton at Cambridge University was to identify genes in mice with diurnal/circadian expression patterns. Tissues from wild type mice were harvested every 3 hrs over a 36 hour time period, for a total of 13 time points. Striatal, cortical, hypothalamic and liver samples were harvested, flash frozen, and transferred to Expression Analysis for RNA extraction and mRNA-seq sequencing. Samples passing QC were analyzed to produce high quality, 50-base paired-end RNA-seq results. Striatal, cortical, hypothalamic and liver samples were collected from 26-week-old wild type C57BL/6J male mice every 3 hours for 36 hours, number of replicates 2 to 12 for each ZT group. mRNA-Seq was performed.

本项目由CHDI基金会与剑桥大学珍妮·莫顿教授合作推进，其核心目标之一是鉴定小鼠体内具有昼夜节律（diurnal/circadian）表达模式的基因。研究人员在36小时的周期内每3小时采集一次野生型小鼠的组织样本，共计13个时间点；采集的组织包括纹状体、皮层、下丘脑与肝脏，经快速冷冻后送至Expression Analysis公司完成RNA提取与mRNA测序（mRNA-seq）。通过质量控制（QC）的样本经分析后，得到了高质量的50碱基双端RNA测序（RNA-seq）结果。本次实验采用26周龄的野生型C57BL/6J雄性小鼠，同样在36小时内每3小时采集一次上述四类组织，每个授时相（ZT）组的生物学重复数为2至12份，随后完成了mRNA测序。