Comparisons between fold-change in gene expression determined by microarray and qRT-PCR methods.

The fold changes in mRNA levels in diabetes (DM) and DM+SERCA2a samples of selected genes were determined by microarray and RT-qPCR. Transcripts were selected based on their roles in insulin signaling, energy/metabolism, Ca2+ handling, structural remodeling and intracellular signaling. CK, creatine kinase; sFRP4, secreted frizzled-related protein 4; Retnla, resistin-like alpha; Cacna1c, calcium channel, voltage-dependent, L type, alpha 1C subunit; Cacnb3, calcium channel, voltage-dependent, beta 3 subunit. N/A, not amplified.

本研究通过微阵列（microarray）与实时定量聚合酶链反应（RT-qPCR）测定了糖尿病（DM）组及DM+SERCA2a组中选定基因的mRNA水平折叠变化。转录本的筛选依据为其在胰岛素信号通路、能量/代谢、钙离子处理（Ca2+ handling）、结构重塑以及细胞内信号转导中的作用。CK即肌酸激酶（creatine kinase）；sFRP4为分泌型卷曲相关蛋白4（secreted frizzled-related protein 4）；Retnla为抵抗素样α（resistin-like alpha）；Cacna1c为电压依赖性L型钙通道α1C亚基（calcium channel, voltage-dependent, L type, alpha 1C subunit）；Cacnb3为电压依赖性钙通道β3亚基（calcium channel, voltage-dependent, beta 3 subunit）。N/A表示未扩增（not amplified）。