Glomerular transcriptomic analysis of the influence of Genetic background effect during anti-GBM glomerulonephritis in mice

We analyzed the impact of the genetic background during experimental passive non-accelerated anti-glomerular basement membrane glomerulonephritis (anti-GBM-GN) (an equivalent to nephrotoxic nephritis) in two different mouse genetic backgrounds (C57BL6/J vs 129S2svPAS/crl). Glomerular extracts have been made at an early time point (e.g day 4 of the induction of the glomerulonephritis). RNA have been extracted following standard procedures. 3 mice per group have been compared. 3 Control mice from each background. And 3 anti-GBM-GN challenged mice from each Genetic background, corresponding to 12 samples in total.

本研究分析了两种不同小鼠遗传背景（C57BL6/J与129S2svPAS/crl）下，被动非加速型实验性抗肾小球基底膜肾小球肾炎（anti-glomerular basement membrane glomerulonephritis, anti-GBM-GN，其等价于肾毒性肾炎（nephrotoxic nephritis））模型中遗传背景的影响效应。我们于肾炎诱导早期（例如造模第4天）制备肾小球提取物，并依照标准流程提取RNA。实验分组如下：每种遗传背景各设置3只空白对照小鼠，以及3只经anti-GBM-GN造模攻击的小鼠，总计共12个样本。