[Adipose-derived stem cells in the proliferation phase of the healing process in cold burns]

ABSTRACT Cryosurgery has been used to treat different diseases of systems and organs, although adverse effects have been reported such as delayed wound healing, large scars, esthetical deformation and functional impairment. Injuries caused naturally by the exposure to extreme cold weather conditions mostly result in gangrene. This study aims to evaluate the influence of adipose-derived stem cells (ADSCs) in the proliferation phase on cutaneous wound healing. Through the application of liquid nitrogen by the spraying technique, a 15 millimeter diameter lesion was produced in the dorsal region of each rat. The wound received treatment according to the group it belonged: 1) ADSCs application on the 15th day (treated group); 2) application of 0.9% sodium chloride solution on the 15th day (sham group); 3) no intervention until euthanasia (control group). The group treated with ADSCs showed the highest wound average contraction rate; this group got a significant statistical difference in relation to the sham group when it refers to neovascularization. The ADSCs therapy provides an important clinical evolution of wounds. This was verified at the end of the evaluation period through narrower and longer scars.

摘要 冷冻手术已被用于治疗多系统及器官的多种疾病，但已有报道显示其可引发多种不良反应，包括伤口愈合延迟、瘢痕增生、美观畸形及功能障碍。自然暴露于极端低温环境所致的损伤多会引发坏疽。本研究旨在评估脂肪来源干细胞（adipose-derived stem cells, ADSCs）在细胞增殖期对皮肤伤口愈合的影响。通过喷雾法施用液氮，在每只大鼠背部制备直径15毫米的创面。根据实验分组对伤口进行干预：1）第15天予以ADSCs治疗的治疗组；2）第15天予以0.9%氯化钠溶液的假手术组；3）直至安乐处死前不予任何干预的对照组。实验结果表明，ADSCs治疗组的伤口平均收缩率最高；该组在新生血管生成方面与假手术组存在显著统计学差异。ADSCs疗法可显著改善伤口的临床转归，在评估周期结束时，该组瘢痕表现为更窄更长的特征。