RNA-seq of mouse small intestinal organoids with an intestinal-epithelial specific deletion of LSD1 compared to wild type. RNA-seq of mouse small intestinal organoids with an intestinal-epithelial specific deletion of LSD1 compared to wild type

To assess the role of LSD1 in mouse small intestinal epithelium, we grew small intestinal organoids in vitro from mice with an epithelial specific deletion of LSD1 (Villin-Cre+; Lsd1f/f) and from wild type (Villin-Cre-; Lsd1f/f) mice. This experiment uses a new Cre strain with 100% recombination efficiency. Similar to intestinal epithelium from mice with an intestinal epithelium specific LSD1-KO, Paneth cells are not present in LSD1-KO small intestinal organoids. We used these sequencing data to show intrinsic epithelial changes in the intestinal epithelium caused by LSD1 deletion in the absence of microbiota and surrounding in vivo cell types.

为评估赖氨酸特异性去甲基化酶1（LSD1）在小鼠小肠上皮中的功能，我们分别从上皮细胞特异性敲除LSD1的小鼠（Villin-Cre+; Lsd1f/f）与野生型（Villin-Cre-; Lsd1f/f）小鼠中体外培养小肠类器官。本实验采用了重组效率达100%的新型Cre工具株。与小肠上皮细胞特异性LSD1敲除小鼠的肠上皮表型一致，LSD1敲除的小肠类器官中无潘氏细胞（Paneth cells）存在。我们利用本次测序数据，揭示了在缺乏微生物群及体内周围细胞类型的环境中，LSD1缺失所引发的肠上皮内源性改变。