Chromatin Decondensation Data of SREBP2(N) Overexpression in Human Umbilical Vein Endothelial Cells

Sterol regulatory element-binding protein 2 (SREBP2) is a transcription factor that has been recently discovered to mediate vascular endothelial cell (EC) dysfunction. We therefore investigated the role of SREBP2 in the epigenetic function of EC biology through comparing ATAC-seq of human umbilical vein endothelial cells (HUVECs) that were infected with adenovirus overexpressing SREBP2 (Ad-SREBP2) or with empty vector (Ad-null) control. Gene ontology analysis revealed that SREBP2 not only decondenses chromatin for cholesterol biosynthesis, but also mediates the TGF pathway. Among the responsive promoter, SREBP2 exhibited the induction of genes related to mesenchymal transition. ATAC-seq data of HUVECs overexpressing SREBP2 using adenovirus followed by deep sequencing, in biological duplicate, using Illumina HiSeq 2000.

固醇调节元件结合蛋白2（Sterol regulatory element-binding protein 2，SREBP2）是一类新近被发现可介导血管内皮细胞（vascular endothelial cell，EC）功能异常的转录因子。本研究通过对感染过表达SREBP2的腺病毒（Ad-SREBP2）或空载体对照（Ad-null）的人脐静脉内皮细胞（human umbilical vein endothelial cells，HUVECs）进行转座酶可及性染色质测序（ATAC-seq）比较分析，探究了SREBP2在血管内皮细胞生物学的表观遗传功能中的作用。基因本体分析结果显示，SREBP2不仅可解聚染色质以参与胆固醇生物合成过程，还可介导TGF通路。在响应性启动子区域中，SREBP2可诱导与间质转化相关的基因表达。本数据集包含经腺病毒介导SREBP2过表达的人脐静脉内皮细胞的ATAC-seq数据，该实验设置生物学重复，采用Illumina HiSeq 2000平台完成深度测序。