Harr Gupta et al. raw data

Bleomycin was intratracheally delivered to mouse lungs to induce fibrosis. At different time points post-bleomycin, precision-cut lung slices (PCLS) from fibrotic mouse lungs were incubated with radiolabeled peptide ([64Cu]Cu-PEG-FUD) and radioactivity was quantified via gamma counting and autoradiography. Alternatively, at different time points post-bleomycin, fluorescently labeled peptide (Cy5-PEG-FUD) was delivered to mice subcutaneously, followed by relevant organ ex vivo IVIS fluorescence imaging and signal quantification. Additionally, lung sections were stained with Mason's trichrome staining and subsequently subjected to Ashcroft scoring to determine the level of fibrotic burden.

将博莱霉素（Bleomycin）经气管内递送至小鼠肺部以诱导肺纤维化。在博莱霉素给药后的不同时间点，采集来自纤维化小鼠肺部的精密切割肺切片（precision-cut lung slices, PCLS），将其与放射性标记肽([64Cu]Cu-PEG-FUD)共孵育后，通过γ计数与放射自显影对放射性活度进行定量。另一方面，在博莱霉素给药后的不同时间点，将荧光标记肽(Cy5-PEG-FUD)经皮下注射至小鼠体内，随后对获取的相关器官开展离体IVIS荧光成像，并对成像信号进行定量分析。除此之外，对肺部组织切片进行马森三色染色（Mason's trichrome staining），随后通过Ashcroft评分（Ashcroft scoring）评估纤维化负荷水平。