Effects of amiloride in lithium induced kidney interstitial fibrosis. Effects of amiloride in lithium induced kidney interstitial fibrosis

Purpose: The goal of this study is to understand the role of co-administred amiloride. This was derived through RNAseq of kidney cortex and determining differential genes in response to lithium, lithium+amiloride and control at 14 days, 28 days and 6 months. Methods: RNAseq of kidney cortex treated with either lithium alone, lithium+amiloride or control. Deseq2 was performed using contrast argument with Wald test to identify significant differentially expression genes at each time point individually. Result & Conclusion: Co-administration with amiloride at all time points contributed in the reduction of inflammation and fibrosis caused by lithium. Overall design: mRNA profile of rats treated with lithium compared to lithium, lithium-amiloride compared to lithium at 14 days, 28 days and 6 months time points.

研究目的：本研究旨在探究联合给药阿米洛利（amiloride）的生物学作用。本数据集通过对大鼠肾脏皮质进行RNA测序（RNAseq），分析14天、28天及6个月三个时间点下，分别经单一锂剂、锂剂+阿米洛利处理及对照组样本的差异基因表达情况。
实验方法：对经单一锂剂、锂剂+阿米洛利及对照处理的肾脏皮质样本开展RNA测序（RNAseq）。采用DESeq2工具，以对比参数结合沃尔德检验（Wald test），分别在各时间点下筛选具有显著统计学差异的表达基因。
结果与结论：在所有观测时间点中，联合使用阿米洛利均可有效减轻锂剂诱导的炎症与纤维化反应。
整体实验设计：对比14天、28天及6个月三个时间点下，锂剂处理组与对照组的mRNA表达谱，以及锂剂+阿米洛利处理组与单一锂剂处理组的mRNA表达谱。