Transcriptional profile Lactobacillus plantarum WCFS1 : Hydroxytyrosol treated cells

Whole genome transcriptional profiling was used to characterize the response of Lactobacillus plantarum WCFS1 human isolate during challenge with hydroxytyrosol. Twelve independent experiments were performed and mixed at random in groups of four for total of three RNA samples. Whole genome transcriptional profiling was used to characterize the response of Lactobacillus plantarum WCFS1 human isolate during challenge with hydroxytyrosol. Twelve independent experiments were performed and mixed at random in groups of four for total of three RNA samples. The transcriptional profile reveals the induction of genes related to defense mechanisms against oxidative challenge. This response include genes related to inactivation of toxic oxygen radicals, probably derived from the autooxidation of hydroxytyrosol and genes involved in repairing the damage of oxygen radicals on proteins, including those involved in the metabolism of sulphur amino acids. Genes involved in peptidoglycan turnover, strenghthening of the cell wall and genes coding for osmoprotectants transporters, were downregulated. In addition, genes coding for elements typically involved in the stringent response, including ribosomal proteins, enzymes involved in the metabolism of the ppGpp alarmone, nitrogen metabolism, cell division and general stress proteins, were differentially regulated by hydroxytyrosol. Biological triplicates: untreated cells: Control vs. Hydroxytyrosol treated cells: Experimental

采用全基因组转录谱（Whole genome transcriptional profiling）对人类分离株植物乳杆菌WCFS1（Lactobacillus plantarum WCFS1）在羟基酪醇（hydroxytyrosol）胁迫下的应答特征进行表征。共开展12次独立实验，将其随机以4次实验为一组进行混合，最终获得3份RNA样本。采用全基因组转录谱（Whole genome transcriptional profiling）对人类分离株植物乳杆菌WCFS1（Lactobacillus plantarum WCFS1）在羟基酪醇（hydroxytyrosol）胁迫下的应答特征进行表征。共开展12次独立实验，将其随机以4次实验为一组进行混合，最终获得3份RNA样本。该转录谱揭示了针对氧化胁迫的防御机制相关基因的诱导表达。该应答涉及与有毒氧自由基灭活相关的基因——这类自由基可能源自羟基酪醇的自氧化——以及负责修复氧自由基对蛋白质造成损伤的基因，其中包括参与含硫氨基酸代谢的基因。参与肽聚糖周转、细胞壁加固的基因，以及编码渗透压保护蛋白转运体的基因均出现表达下调。此外，参与严紧反应（stringent response）的典型编码基因——包括核糖体蛋白、ppGpp警报素（ppGpp alarmone）代谢相关酶、氮代谢相关酶、细胞分裂相关蛋白以及通用应激蛋白——均受羟基酪醇调控发生差异表达。生物学重复设置如下：未处理细胞为对照组，经羟基酪醇处理的细胞为实验组。