Blastocyst Formation. Bos taurus

In this study we aimed to examine the effect of alternative in vivo and in vitro culture conditions during the time of blastocyst formation on the transcriptome profile of bovine blastocysts. Overall design: Two different blastocyst groups were produced. The first group (Vitro_morula) was matured, fertilized and cultured in vitro until morula stage then transferred to synchronized recipients and blastocysts were collected at day 7 by uterine flushing. The second group (Vivo_morula) was matured, fertilized and cultured in vivo until morula stage then flushed out and cultured in vitro until day 7. Complete in vitro (IVP) and in vivo blastocysts were produced and used as controls.

本研究旨在探究囊胚形成阶段不同体内（in vivo）与体外（in vitro）培养条件对牛囊胚转录组图谱的影响。
实验设计概述：本研究构建了两组不同的囊胚样本。第一组（Vitro_morula）：先在体外完成卵母细胞成熟、受精及胚胎培养至桑椹胚（morula）阶段，随后将胚胎移植至同步化受体母牛体内，于妊娠第7天通过子宫冲洗法收集囊胚。第二组（Vivo_morula）：先在体内完成卵母细胞成熟、受精及胚胎培养至桑椹胚阶段，随后冲出胚胎并在体外继续培养至第7天。此外，本研究同时制备纯体外培养（IVP，in vitro production）与纯体内发育的囊胚作为对照样本。