Comparative Gene Expression During Sinoatrial Node and Atrial Development

To determine pathways activated during sinoatrial node morphogenesis we conducted bulk RNA sequencing on functionally validated tissue preps of embryonic sinoatrial node and atria. Overall design: Gene expression analysis was preformed on RNAseq data obtained from 3 pooled samples of sinoatrial node tissue and 3 pooled samples of atrial tissue isolated HH stage 30 chick hearts (each sample pool was assembled from ~20 indivudal hearts)

为探究窦房结（sinoatrial node）形态发生过程中激活的信号通路，我们对经功能验证的胚胎窦房结与心房组织样品开展了批量RNA测序（bulk RNA sequencing）。
实验整体设计：本研究针对分离自HH30期鸡胚心脏的3份窦房结组织混合样本与3份心房组织混合样本的RNA测序数据开展基因表达分析，每份混合样本由约20个独立鸡胚心脏组装制备而成。