Expression of candidate genes by keratinocytes co-cultured with skin commensal

To investigate whether skin bacteria might influence the expression of selected genes, we co-cultured human keratinocytes with S. epidermidis, an abundant commensal in human skin and performed RNA sequencing analysis. Overall design: Keratinocytes were cultured with and without the addition of Staphylococcus epidermidis cultures, six replicates in each group. Host RNA were isolated and RNA-Seq was performed to identify differential expression of selected genes.

为探究皮肤细菌是否会影响特定基因的表达，我们将人类角质形成细胞与表皮葡萄球菌（S. epidermidis）——一种人体皮肤中丰度较高的共生菌——进行共培养，并开展了RNA测序（RNA sequencing）分析。
实验设计概述：将角质形成细胞分为两组，一组添加表皮葡萄球菌培养液，另一组不添加，每组设置6个生物学重复。提取宿主RNA并进行RNA-Seq测序，以鉴定目标基因的差异表达情况。