Data from: Effects of parasitic sex-ratio distorters on host genetic structure in the Armadillidium vulgare-Wolbachia association

In the pill bug Armadillidium vulgare (Crustacea, Oniscidea) Wolbachia facilitates its spread through vertical transmission via the eggs by inducing feminization of genetic males. The spread of feminizing Wolbachia within and across populations is therefore expected to influence mtDNA genetic structure by hitchhiking. To test this hypothesis, we analysed nuclear and mtDNA genetic structure, and Wolbachia prevalence in 13 populations of the pill bug host. Wolbachia prevalence (ranging from 0 to 100% of sampled females) was highly variable among populations. All three Wolbachia strains previously observed in A. vulgare were present (wVulC, wVulM and wVulP) with wVulC being the most prevalent (9 out of 13 populations). The host showed a genetic structure on five microsatellite loci that is compatible with isolation by distance. The strong genetic structure observed on host mtDNA was correlated to Wolbachia prevalence: three mitotypes were in strong linkage disequilibrium with the three strains of Wolbachia. Neutrality tests showed that the mtDNA polymorphism is not neutral and we thus suggest that this unusual pattern of mtDNA polymorphism found in A. vulgare was due to Wolbachia.

在卷球鼠妇（Armadillidium vulgare，隶属于甲壳纲等足目）中，沃尔巴克氏体（Wolbachia）可通过诱导遗传雄性发生雌性化，经卵垂直传播实现自身的种群扩散。因此，具备雌性化能力的沃尔巴克氏体在种群内部及跨种群的扩散，有望通过遗传搭车效应改变宿主线粒体DNA（mtDNA）的遗传结构。为验证这一假说，我们对13个卷球鼠妇宿主种群的核基因组与线粒体DNA遗传结构，以及沃尔巴克氏体感染率展开了分析。结果显示，各宿主种群间的沃尔巴克氏体感染率差异显著，采样雌性个体的感染率范围为0至100%。本研究共检测到此前在卷球鼠妇中已报道的全部3种沃尔巴克氏体菌株（wVulC、wVulM与wVulP），其中wVulC为优势菌株，在13个种群中的9个种群均能检测到该菌株。宿主在5个微卫星位点上的遗传结构符合距离隔离模式。我们观测到的宿主线粒体DNA强遗传结构与沃尔巴克氏体感染率呈显著相关：3种线粒体单倍型分别与3种沃尔巴克氏体菌株存在强烈的连锁不平衡。中性检验结果表明，该线粒体DNA多态性并非遵循中性演化模式。据此我们推测，卷球鼠妇中这种非典型的线粒体DNA多态性格局，正是由沃尔巴克氏体介导所形成的。