Effect of mitochondria deficiency on senescence-associated gene expression

We used parkin –overexpressing MRC5 fibroblasts to investigate the role of mitochondria deficiency on senescence-associated gene expression. Overall design: RNA-seq analysis on proliferating and senescent Parkin-expressing MRC5 fibroblasts treated with CCCP (treated) or DMSO (Untreated).

本研究采用过表达帕金蛋白（Parkin）的MRC5成纤维细胞，探究线粒体缺陷对衰老相关基因表达的调控作用。整体实验设计：对经CCCP处理或二甲基亚砜（DMSO，未处理组）处理的增殖型与衰老型过表达帕金蛋白的MRC5成纤维细胞开展RNA测序（RNA-seq）分析。