A survey of human brain transcriptome diversity at the single cell level

We used single cell RNA sequencing on 466 cells to capture the cellular complexity of the adult and fetal human brain at a whole transcriptome level. Healthy adult temporal lobe tissue was obtained from epileptic patients during temporal lobectomy for medically refractory seizures. We were able to classify individual cells into all of the major neuronal, glial, and vascular cell types in the brain. Examination of cell types in healthy human brain samples.

本研究采用单细胞RNA测序（single cell RNA sequencing）技术对466个细胞进行测序，旨在从全转录组层面解析成人与胎儿人类大脑的细胞复杂性。健康成人颞叶组织取自药物难治性癫痫患者行颞叶切除术时的手术标本。本研究可将单个细胞归类为大脑内所有主要的神经元、神经胶质及血管细胞类型。本研究同时对健康人类大脑样本的细胞类型进行了考察分析。