Expression analysis of wild-type and transcription factor mutants in response to rapamycin. Saccharomyces cerevisiae

Transcript level changes in transcription factor mutants after rapamycin treatment, compared to the wild-type strain. This data set accompanies the study "Widespread Misinterpretable ChIP-seq Bias in Yeast" (GSE51251) Overall design: Four separate wild-type cultures of yeast were grown, and each culture was split into two. Then, one was treated with DMSO and the other one was treated with rapamycin. For transcription factor mutants, two separate cultures were grown, and rapamycin was treated in the same way as treated to the wild type. Total RNA was recovered from each culture, then labeled oligo was prepared following NimbleGene standard protocol. Each chip measures the expression levels of 5,777 genes from yeast.

本数据集聚焦雷帕霉素处理后转录因子突变株与野生型菌株的转录本水平变化。本数据集配套于研究《酿酒酵母中广泛存在的易被误解的ChIP-seq偏好性》（GSE51251）。实验设计概况：分别培养4组独立的酿酒酵母野生型菌株，将每一组培养液均分为两份，其中一份用二甲基亚砜（DMSO）处理，另一份用雷帕霉素处理；对于转录因子突变株，同样培养2组独立培养液，并以与野生型一致的方式施加雷帕霉素处理。从各组培养液中回收总RNA，随后按照NimbleGene标准流程制备标记寡核苷酸探针；每一张芯片可检测酿酒酵母5777个基因的表达水平。