Genome-wide DNA methylation maps of histone deacetylase inhibition in triple-negative breast cancer. Homo sapiens

Analysis of DNA methylation changes after prolonged exposure of triple-negative breast cancer cell lines to low doses of Panobinostat (LBH589), a pan-histone deacetylase inhibitor. Overall design: Bisulfite-converted DNA was hybridized to the Illumina Infinium 450k Human Methylation BeadChip to generate genome-wide DNA methylation maps of triple-negative cell lines cell types after histone deacetylase inhibition.

本研究旨在分析三阴性乳腺癌细胞系长期暴露于低剂量帕比司他（Panobinostat, LBH589，一种泛组蛋白去乙酰化酶抑制剂）后所产生的DNA甲基化变化。总体实验设计：将经亚硫酸氢盐转化的DNA与Illumina Infinium 450k人类甲基化微珠芯片进行杂交，以生成三阴性乳腺癌细胞系在组蛋白去乙酰化酶抑制处理后的全基因组DNA甲基化图谱。