Gene expression of three hepatocellular carcinoma cell lines with different organ-tropism.

In this study we investigated the gene expression profiling in three HCC cell lines with different organ-tropism.The parent cell line has low metastasis ability in nude mice model. Subclone 1 has higher metastasis ability specific to lung, and subclone 2 has dual metastasis ability to lung and celiac lymph node. We aimed to explore differentially expressed genes involved in process of HCC metastasis and organ-specific metastasis, and identify their biological functions. Overall design: Examination of different gene expression among the 3 cell types.

本研究针对3株具有不同器官嗜性的肝细胞癌（Hepatocellular Carcinoma, HCC）细胞系开展基因表达谱分析。其中，亲本细胞系在裸鼠模型中转移能力较低；亚克隆1具备肺特异性高转移能力，亚克隆2则对肺与腹腔淋巴结兼具双重转移能力。本研究旨在探究参与肝细胞癌转移及器官特异性转移过程的差异表达基因（differentially expressed genes），并明确其生物学功能。整体设计：对3种细胞类型的基因表达差异进行检测。