Table_2_Comprehensive Transcriptomic and Metabolomic Analysis of the Litopenaeus vannamei Hepatopancreas After WSSV Challenge.xlsx

Litopenaeus vannamei is the major farmed shrimp species worldwide. White spot disease due to white spot syndrome virus (WSSV) is severely affecting shrimp worldwide, causing extensive economic losses in L. vannamei culture. This is the first study that applied combined transcriptomic and metabolomic analysis to study the effects on the L. vannamei hepatopancreas after WSSV challenge. Our transcriptomic data revealed differentially expressed genes (DEGs) associated with immunity, apoptosis, the cytoskeleton and the antioxidant system in the hepatopancreas of L. vannamei. Metabolomic results showed that WSSV disrupts metabolic processes including amino acid metabolism, lipid metabolism and nucleotide metabolism. After challenged by WSSV, immune-related DEGs and differential metabolites (DMs) were detected in the hepatopancreas of L. vannamei, indicating that WSSV may damage the immune system and cause metabolic disorder in the shrimp. In summary, these results provide new insights into the molecular mechanisms underlying L. vannamei’s response to WSSV.

凡纳滨对虾（Litopenaeus vannamei）是全球范围内的主要养殖虾类。由白斑综合征病毒（White Spot Syndrome Virus, WSSV）诱发的白斑病，对全球虾类养殖业造成严重危害，给凡纳滨对虾的养殖产业带来了巨大经济损失。本研究首次采用转录组学与代谢组学联合分析的方法，探究白斑综合征病毒（WSSV）侵染后凡纳滨对虾肝胰腺的响应变化。转录组数据显示，凡纳滨对虾肝胰腺中存在与免疫、细胞凋亡、细胞骨架及抗氧化系统相关的差异表达基因（differentially expressed genes, DEGs）。代谢组学分析结果表明，白斑综合征病毒会干扰虾体内的氨基酸代谢、脂代谢与核苷酸代谢等多种代谢过程。经白斑综合征病毒侵染后，凡纳滨对虾肝胰腺中可检测到免疫相关差异表达基因与差异代谢物（differential metabolites, DMs），提示白斑综合征病毒可能损伤虾类免疫系统并引发代谢紊乱。综上，本研究结果为解析凡纳滨对虾应对白斑综合征病毒侵染的分子机制提供了新的研究视角。