mRNA expression analysis of radiation-induced skin fibrosis of rats

This study aims to compared mRNA expression between radiation-induced fibrotic skin and adjacent normal tissues of rats by RNA-Seq. Male Sprague-Dawley (SD) rats (4 weeks old) were irradiated with a single 45-Gy dose of irradiation was administered to the treatment area at a rate of 750 cGy/min using a 6-MeV electron beam accelerator (Clinac 2100EX, Varian Medical Systems, Inc., CA). Skin tissues from nonirradated skin areas and irradietd areas were collected and subjected to mRNA expression analysis.本研究旨在通过RNA测序（RNA-Seq）比较大鼠放射性纤维化皮肤与其邻近正常组织的mRNA表达水平。选取4周龄的雄性斯普拉-道来（Sprague-Dawley, SD）大鼠，采用6 MeV电子束加速器（Clinac 2100EX，瓦里安医疗系统公司，美国加利福尼亚州），以750 cGy/min的剂量率向治疗区域单次给予45 Gy的射线照射。采集未照射区域与照射区域的皮肤组织，开展mRNA表达分析。