Temporal chromatin accessibility changes define transcriptional states essential for osteosarcoma metastasis [143b_ATACseq]. Temporal chromatin accessibility changes define transcriptional states essential for osteosarcoma metastasis [143b_ATACseq]

ATAC-seq was performed on 143b-GFP cells in vitro or isolated from mouse lung 1 day or 22 days post-innoculation to assess chromatin accessbility dynamics during lung colonization. Overall design: Comparison of chromatin accessibility between 143b-GFP cells grown in vitro or at two timepoints during lung colonization. For the in vivo conditions, mouse lungs were dissociated using the Miltenyi human tumor dissociation kit and mouse cell depletion kit.

本研究通过转座酶可及性测序（ATAC-seq）技术，对体外培养的143b-GFP细胞，以及经小鼠肺部接种后1天或22天分离得到的143b-GFP细胞进行检测，以评估肺部定植过程中的染色质开放动态变化。整体实验设计：对比体外培养的143b-GFP细胞与肺部定植两个时间点的143b-GFP细胞之间的染色质开放差异。针对体内实验组，小鼠肺部采用美天旎（Miltenyi）人类肿瘤解离试剂盒与小鼠细胞耗竭试剂盒进行解离处理。