Gene expression profiles for porcine alveolar macrophage isolated from piglets injected with Mab followed by challenge with two PRRSV strains: PRRSV-JXA1 and PRRSV-HNhx

An PRRSV specific IgG-type Mab-PN9cx3, demonstrated broad recognition and neutralization activity against both PRRSV-1 and PRRSV-2 isolates in vitro, was tested for in vivo protection experiments. Administration of Mab-PN9cx3 20mg in piglets significantly alleviated the pathological changes in lungs and decreased virus loads in PAMs after challenging with two heterogenous PRRSV isolates: HP-PRRSV-JXA1 and PRRSV NADC-30 like HNhx when compared with piglets inoculated virus . Transcriptome profile for porcine alveolar macrophage of different groups were analyzed via RNA-sequencing. PAMs isolated from different groups (n=3) were harvested by TRIzol for RNA extraction and subjected to RNA-sequencing

一株猪繁殖与呼吸综合征病毒（Porcine Reproductive and Respiratory Syndrome Virus, PRRSV）特异性IgG型单克隆抗体（Monoclonal Antibody, Mab）PN9cx3，经体外实验证实可广谱识别并中和PRRSV-1与PRRSV-2的多种分离株，随后被用于体内保护试验。给仔猪注射20mg Mab-PN9cx3后，与仅接种病毒的仔猪相比，用两株异源PRRSV分离株——高致病性PRRSV-JXA1（HP-PRRSV-JXA1）和类PRRSV NADC-30的HNhx毒株——攻毒的仔猪，其肺部病理损伤显著缓解，肺泡巨噬细胞（Porcine Alveolar Macrophage, PAM）内的病毒载量也显著降低。本研究通过RNA测序（RNA-sequencing）分析了不同组仔猪的肺泡巨噬细胞转录组图谱。采集不同组（n=3）分离得到的猪肺泡巨噬细胞（PAMs），采用TRIzol法提取总RNA并进行RNA测序分析。