Transcriptomic in silico screens identify niclosamide for protection against cisplatin- and noise-induced hearing loss in zebrafish and mice through Nrf2 activation

We report the RNA expression of the House Ear Institute-Organ of Corti (HEI-OC1) cells with and without cisplatin exposure to identify differencialyy expressed genes. The results of RNA-seq analysis showed 2732 downregulated, and 1639 upregulated genes above 1-fold change in cisplatin threated cells compared to non-treated controls. RNA-seq analysis of HEI-OC1 cells following 15-hour exposure to cisplatin (70 µM)

本研究报道了经顺铂处理与未处理的豪斯耳研究所耳蜗管细胞（House Ear Institute-Organ of Corti，HEI-OC1）的RNA表达谱，旨在筛选差异表达基因。RNA测序（RNA-seq）分析结果显示，与未处理对照组相比，顺铂处理的细胞中存在2732个下调基因、1639个上调基因，其表达倍数变化均大于1倍。本研究针对暴露于70 μM顺铂环境15小时后的HEI-OC1细胞开展了RNA测序分析。