Transcriptomics of Marchantia polymorpha that ectopically expresses MpGL3

An understanding of the evolutionary aspects of gene regulatory network (GRN) is fundamental to elucidate mechanisms of environmental adaptations; nevertheless, how the GRNs leading to land adaptation evolved remains poorly understood. In this study, we isolated a single IIIf bHLH TF from M. polymorpha, MpGL3. Transgenic M. polymorph ectopically expressing MpGL3 (referred to MpGL3ox) decreased the size and numbers of gemma cups, suggesting that MpGL3 is involved in the regulation of morphological development. Transcriptomics analysis of MpGL3ox was performed. We made transgenic M. polymorpha that carries MpGL3 fused to citrione driven by constitutive expression promoter. We performed RNA-Seq of two independent lines and compared with wild type (Tak-1) with biologically triplicates.

基因调控网络（gene regulatory network, GRN）的演化特征解析，是阐明环境适应机制的核心基础；然而，介导陆生适应的基因调控网络的演化路径仍知之甚少。本研究从地钱（Marchantia polymorpha，缩写M. polymorpha）中分离得到单个IIIf亚家族bHLH转录因子（transcription factor, TF）MpGL3。异位表达MpGL3的转基因地钱株系（简称MpGL3ox）的胞芽杯尺寸与数量均出现下降，表明MpGL3参与形态发育调控。本研究对MpGL3ox株系开展了转录组学分析。我们构建了由组成型表达启动子驱动、融合有citrione标签的MpGL3转基因地钱，选取2个独立株系进行RNA测序（RNA-Seq），并设置3次生物学重复，与野生型株系Tak-1进行对照分析。