Identification of the RB loss-induced E2F1 cistrome in prostate cancer [ChIP-seq]

The retinoblastoma protein (RB) is preferentially lost in the progression to castrate resistant prostate cancer (CRPC). However, the alterations associated with such loss have been scantly described. The current study aims to provide molecular mechanisms underlying Rb loss-driven CRPC phenotypes. Alterations in E2F1 binding upon RB loss were assessed in hormone deficient conditions through ChIP-Seq of shCON and shRB LNCaP prostate cancer cells.

视网膜母细胞瘤蛋白（retinoblastoma protein, RB）在进展至去势抵抗性前列腺癌（castrate resistant prostate cancer, CRPC）的过程中优先发生缺失。然而，与该缺失相关的分子改变尚未得到充分阐释。本研究旨在阐明RB缺失驱动CRPC表型的潜在分子机制。研究通过对转染对照短发卡RNA（shCON）与RB靶向短发卡RNA（shRB）的LNCaP前列腺癌细胞开展染色质免疫共沉淀测序（ChIP-Seq），评估了激素缺乏条件下RB缺失所导致的E2F1结合位点改变。